Why do the pvc plastic wells used in this weeks elisa need to be sticky on the inner walls and how may your results change if a different no

Question

Why do the pvc plastic wells used in this weeks elisa need to be sticky on the inner walls and how may your results change if a different non-adherent plastic was used instead?

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Thu Hương 4 years 2021-09-03T12:31:51+00:00 1 Answers 3 views 0

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  1. King
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    2021-09-03T12:33:19+00:00
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    Answer:

    The stickiness in the inner walls allows them to be easily coated with the desired antigens, this translates in the use of a smaller amount of antigen. If the walls weren’t sticky there’s a possibility the antigen won’t stick to them and therefore the result of the ELISA can be a false negative.

    I hope you find this information useful and interesting! Good luck!

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