## 1.Using the absorbance of the spinach extract and the equation of the trendline, determine the concentration of the extract solution. Report

Question

1.Using the absorbance of the spinach extract and the equation of the trendline, determine the concentration of the extract solution. Report the concentration in moles/L (M).
2. Calculate the number of rams of chlorophyll-a in the 25ml, spinach solution.
3. Calculate the concentration of the chloropyhll-a soultion in spinach (mg chlorophyll-a/g spinach)
Trendline: y=1609x + .0055
Absorbance spinach extract lamda max: .329
Absorbacne spinach extract, 750 nm: .023
Corrected absorbance: .306
Mass of Spinach: .1876g Total Volume of spinach: 25mL

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6 months 2021-07-13T04:25:36+00:00 1 Answers 12 views 0

## Answers ( )

Explanation:

From the given information:

We are to make use of the spinach absorbance extract which is the corrected absorbance  (y) = 0.306

And also the trendline equation:

y = 1609x + 0.0055

where,

x = absorbance of the spinach extract.

0.306 = 1609x + 0.0055

collecting the like terms

0.306 – 0.0055 = 1609x

0.3005  = 1609x

x = 0.3005/1609

x = 1.8676 × 10⁻⁴

x ≅ 0.0002 M

No. of grams for the chlorophyll can be computed as follows:

recall that:

molar mass of chlorophyll = 893.5 g/mol

the volume = 25ml = (25/1000) L = 0.025 L

In spinach solution, the no. of grams for the chlorophyll:

= (0.0002) mol/L × (893.5 g/mol) × (0.025) L

= 0.0044675 g

≅ 0.0045 g

In the spinach, the concentration of chlorophyll = no of grams of chlorophyll/ mass of the spinach

= 4.5 mg/0.1876 g

= 23.987 mg/g

≅ 24 mg/g